Genotyping and primer details


Genotyping and primer details for 057H09 - At4g02480
Line ID 057H09
Locus At4g02480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1085613 of line 057H09 Chr4:1085613
   
Insertion allele amplicon 8474 - 71gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 71gp: 5'-ATGACCCTCCTTCACTCTCACTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1085025
   
wt amplicon (over InsPos) 20hf - 71gp
wt amplicon start Chr4:1085025
wt amplicon end Chr4:1085929
wt amplicon size 905 bp
Locus specific primer 71gp: 5'-ATGACCCTCCTTCACTCTCACTAA-3'
2nd locus specific primer 20hf: 5'-TGTACTGTTTCTGGATGGTTTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11