Genotyping and primer details


Genotyping and primer details for 648C02 - At4g05140
Line ID 648C02
Locus At4g05140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2651512 of line 648C02 Chr4:2651512
   
Insertion allele amplicon 8474 - 10ih
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10ih: 5'-TATCCAGACTGCTGATGATGTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2651854
   
wt amplicon (over InsPos) 16ji - 10ih
wt amplicon start Chr4:2651013
wt amplicon end Chr4:2651854
wt amplicon size 842 bp
Locus specific primer 10ih: 5'-TATCCAGACTGCTGATGATGTTTC-3'
2nd locus specific primer 16ji: 5'-ACTCAGAAGGCATTTTTCTTACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11