Genotyping and primer details


Genotyping and primer details for 895G12 - At4g13070
Line ID 895G12
Locus At4g13070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7622353 of line 895G12 Chr4:7622353
   
Insertion allele amplicon 8474 - 17wq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17wq: 5'-CCATAAGACCATACATTGGTGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7621536
   
Insertion allele amplicon 8474 - CB07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB07: 5'-TGTGTCTTTCTTTCTGTGTGCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7622966
   
wt amplicon (over InsPos) 09cm - CB07
wt amplicon start Chr4:7621876
wt amplicon end Chr4:7622966
wt amplicon size 1091 bp
Locus specific primer CB07: 5'-TGTGTCTTTCTTTCTGTGTGCTCT-3'
2nd locus specific primer 09cm: 5'-AAGCTCCTTCTCCAGCTTCTCTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11