Genotyping and primer details


Genotyping and primer details for 100H11 - At5g03350
Line ID 100H11
Locus At5g03350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:816130 of line 100H11 Chr5:816130
   
Insertion allele amplicon 8474 - GA10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GA10: 5'-TTGTAGGTGATGCGTTTACTTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:816768
   
Insertion allele amplicon 3144 - GA11
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer GA11: 5'-GTGATGTTTCTGTGTGTCAGGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:815601
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11