Genotyping and primer details


Genotyping and primer details for 215E11 - At5g11100
Line ID 215E11
Locus At5g11100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3532849 of line 215E11 Chr5:3532849
   
Insertion allele amplicon 8474 - IF39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IF39: 5'-AAGTGTGAACAAAAATGGGTTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3532388
   
wt amplicon (over InsPos) 04id - IF39
wt amplicon start Chr5:3532388
wt amplicon end Chr5:3533375
wt amplicon size 988 bp
Locus specific primer IF39: 5'-AAGTGTGAACAAAAATGGGTTTTC-3'
2nd locus specific primer 04id: 5'-AAACTCCTGAAAATGAGTGAGCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11