Genotyping and primer details


Genotyping and primer details for 757A06 - At5g16480
Line ID 757A06
Locus At5g16480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5382208 of line 757A06 Chr5:5382208
   
Insertion allele amplicon 8474 - 26au
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26au: 5'-GGATGATGATAACGATGGAGAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5382604
   
Insertion allele amplicon 8474 - NL72
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NL72: 5'-TGAGACAGCTAAAAGAGAAAAGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5381770
   
wt amplicon (over InsPos) 26au - NL72
wt amplicon start Chr5:5381770
wt amplicon end Chr5:5382604
wt amplicon size 835 bp
Locus specific primer NL72: 5'-TGAGACAGCTAAAAGAGAAAAGCC-3'
2nd locus specific primer 26au: 5'-GGATGATGATAACGATGGAGAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11