Genotyping and primer details


Genotyping and primer details for 011A07 - At5g19830
Line ID 011A07
Locus At5g19830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6703678 of line 011A07 Chr5:6703678
   
Insertion allele amplicon 3144 - 01vo
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 01vo: 5'-TGCTACTGCACTTCTTCTGTTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6703407
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11