Genotyping and primer details


Genotyping and primer details for 143D03 - At5g23070
Line ID 143D03
Locus At5g23070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7742813 of line 143D03 Chr5:7742813
   
Insertion allele amplicon 8474 - FK11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FK11: 5'-AAGAGAAGTAGCCACAAGAAGCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7743348
   
Insertion allele amplicon 3144 - KB67
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KB67: 5'-AATGTTCTCCGGTAAAACAACAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7742233
   
wt amplicon (over InsPos) FK11 - KB67
wt amplicon start Chr5:7742233
wt amplicon end Chr5:7743348
wt amplicon size 1116 bp
Locus specific primer KB67: 5'-AATGTTCTCCGGTAAAACAACAAC-3'
2nd locus specific primer FK11: 5'-AAGAGAAGTAGCCACAAGAAGCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11