Genotyping and primer details


Genotyping and primer details for 356B07 - At5g26010
Line ID 356B07
Locus At5g26010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9086946 of line 356B07 Chr5:9086946
   
Insertion allele amplicon 8474 - AB66
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AB66: 5'-AAATGTACCTGAGTTATGGCAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9086525
   
wt amplicon (over InsPos) 14cd - AB66
wt amplicon start Chr5:9086525
wt amplicon end Chr5:9087361
wt amplicon size 837 bp
Locus specific primer AB66: 5'-AAATGTACCTGAGTTATGGCAACC-3'
2nd locus specific primer 14cd: 5'-CTTTTCGTTACCGTCTTCTCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11