Genotyping and primer details


Genotyping and primer details for 283G07 - At5g27260
Line ID 283G07
Locus At5g27260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9604002 of line 283G07 Chr5:9604002
   
Insertion allele amplicon 8474 - 43vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 43vh: 5'-CATTCGACTGCAAAGAAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9603709
   
wt amplicon (over InsPos) 46vw - 43vh
wt amplicon start Chr5:9603709
wt amplicon end Chr5:9604613
wt amplicon size 905 bp
Locus specific primer 43vh: 5'-CATTCGACTGCAAAGAAAAAG-3'
2nd locus specific primer 46vw: 5'-CATGAGACATAAATGGTGCGTAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11