Genotyping and primer details


Genotyping and primer details for 053H04 - At5g35160
Line ID 053H04
Locus At5g35160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:13416004 of line 053H04 Chr5:13416004
   
Insertion allele amplicon 8474 - 05lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05lu: 5'-CAGATGCTGGACATGATAAAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:13416566
   
wt amplicon (over InsPos) 02mc - 05lu
wt amplicon start Chr5:13415537
wt amplicon end Chr5:13416566
wt amplicon size 1030 bp
Locus specific primer 05lu: 5'-CAGATGCTGGACATGATAAAGAAA-3'
2nd locus specific primer 02mc: 5'-CGACTATTGGGAAGAAAGATTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11