Genotyping and primer details


Genotyping and primer details for 111D02 - At5g41310
Line ID 111D02
Locus At5g41310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16519913 of line 111D02 Chr5:16519913
   
Insertion allele amplicon 3144 - AK46
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AK46: 5'-TGTTTGTTTCTTGTTTTGTCCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16519430
   
wt amplicon (over InsPos) 05nv - AK46
wt amplicon start Chr5:16519430
wt amplicon end Chr5:16520467
wt amplicon size 1038 bp
Locus specific primer AK46: 5'-TGTTTGTTTCTTGTTTTGTCCTTG-3'
2nd locus specific primer 05nv: 5'-TTTATAAACCACCAGACAGAGGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11