Genotyping and primer details


Genotyping and primer details for 545G01 - At5g42170
Line ID 545G01
Locus At5g42170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16850434 of line 545G01 Chr5:16850434
   
Insertion allele amplicon 8474 - 12ib
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12ib: 5'-AAATCCAAAGCTCAAAAATGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16850080
   
wt amplicon (over InsPos) 11io - 12ib
wt amplicon start Chr5:16850080
wt amplicon end Chr5:16850853
wt amplicon size 774 bp
Locus specific primer 12ib: 5'-AAATCCAAAGCTCAAAAATGAAGA-3'
2nd locus specific primer 11io: 5'-TTGGAGATATCTTCGCGTTAAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11