Genotyping and primer details


Genotyping and primer details for 760A07 - At5g44320
Line ID 760A07
Locus At5g44320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17855344 of line 760A07 Chr5:17855344
   
Insertion allele amplicon 8474 - 71ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 71ra: 5'-TGAACCAACATTTTGGAACATAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17854789
   
wt amplicon (over InsPos) 05rj - 71ra
wt amplicon start Chr5:17854789
wt amplicon end Chr5:17855691
wt amplicon size 903 bp
Locus specific primer 71ra: 5'-TGAACCAACATTTTGGAACATAAA-3'
2nd locus specific primer 05rj: 5'-ACTCTGCTCATTCTCTAGGTCTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11