Genotyping and primer details


Genotyping and primer details for 554G10 - At5g48360
Line ID 554G10
Locus At5g48360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19596583 of line 554G10 Chr5:19596583
   
Insertion allele amplicon 8474 - 32fm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32fm: 5'-GAATCTGACCATATTAGAACCGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19596241
   
wt amplicon (over InsPos) 35go - 32fm
wt amplicon start Chr5:19596241
wt amplicon end Chr5:19596893
wt amplicon size 653 bp
Locus specific primer 32fm: 5'-GAATCTGACCATATTAGAACCGGA-3'
2nd locus specific primer 35go: 5'-TTGCTCTGTTGAGAAGAAGATTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11