Genotyping and primer details


Genotyping and primer details for 791F09 - At5g59680
Line ID 791F09
Locus At5g59680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24050332 of line 791F09 Chr5:24050332
   
Insertion allele amplicon 3144 - 77wp
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 77wp: 5'-AAGGCAGAGGTATTTCCAGTACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24049654
   
Insertion allele amplicon 8474 - EU67
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU67: 5'-ACAAATACAAACAGAGCACACGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24050892
   
wt amplicon (over InsPos) BH11 - EU67
wt amplicon start Chr5:24038546
wt amplicon end Chr5:24050892
wt amplicon size 1137 bp
Locus specific primer EU67: 5'-ACAAATACAAACAGAGCACACGAC-3'
2nd locus specific primer BH11: 5'-TGATCTTCTTCTGAGAGTTCACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11