Genotyping and primer details


Genotyping and primer details for 327D05 - At5g65120
Line ID 327D05
Locus At5g65120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26013358 of line 327D05 Chr5:26013358
   
Insertion allele amplicon 8474 - CN50
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CN50: 5'-AAAACACAGAACCCAATTTTTACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26014381
   
wt amplicon (over InsPos) 12si - CN50
wt amplicon start Chr5:26013020
wt amplicon end Chr5:26014381
wt amplicon size 1362 bp
Locus specific primer CN50: 5'-AAAACACAGAACCCAATTTTTACG-3'
2nd locus specific primer 12si: 5'-TCCATTGGTAAGTAACCCAAACTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11