Genotyping and primer details


Genotyping and primer details for 584E02 - At5g65180
Line ID 584E02
Locus At5g65180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26047165 of line 584E02 Chr5:26047165
   
Insertion allele amplicon 8474 - 19vi
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19vi: 5'-AAATTATCAAGTGGAGGTGTGTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26046686
   
wt amplicon (over InsPos) 40vu - 19vi
wt amplicon start Chr5:26046686
wt amplicon end Chr5:26047796
wt amplicon size 1111 bp
Locus specific primer 19vi: 5'-AAATTATCAAGTGGAGGTGTGTCC-3'
2nd locus specific primer 40vu: 5'-TGTGGAGGTCCAAAATACATCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11