Genotyping and primer details


Genotyping and primer details for 297B10 - At5g67060
Line ID 297B10
Locus At5g67060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26766833 of line 297B10 Chr5:26766833
   
Insertion allele amplicon 3144 - 13bd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 13bd: 5'-CCTAATTTACAAACTCGCTCCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26767192
   
Insertion allele amplicon 8474 - PH69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH69: 5'-ACCACAACAACACTTACCCTTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26766364
   
wt amplicon (over InsPos) 13bd - PH69
wt amplicon start Chr5:26766364
wt amplicon end Chr5:26767192
wt amplicon size 829 bp
Locus specific primer PH69: 5'-ACCACAACAACACTTACCCTTTTC-3'
2nd locus specific primer 13bd: 5'-CCTAATTTACAAACTCGCTCCAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11