Genotyping and primer details


Genotyping and primer details for 009H04 - At4g13240
Line ID 009H04
Locus At4g13240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7682171 of line 009H04 Chr4:7682171
   
Insertion allele amplicon 3144 - CB37
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CB37: 5'-GTGGGGGTAACATGTTGTTTTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7682575
   
Insertion allele amplicon 8474 - MF24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MF24: 5'-AACGAGAGTTGTTCGAAGAGAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7681690
   
wt amplicon (over InsPos) CB37 - MF24
wt amplicon start Chr4:7681690
wt amplicon end Chr4:7682575
wt amplicon size 886 bp
Locus specific primer MF24: 5'-AACGAGAGTTGTTCGAAGAGAGAG-3'
2nd locus specific primer CB37: 5'-GTGGGGGTAACATGTTGTTTTACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11