Genotyping and primer details


Genotyping and primer details for 011F07 - At1g23310
Line ID 011F07
Locus At1g23310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8268938 of line 011F07 Chr1:8268938
   
Insertion allele amplicon 3144 - BJ03
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer BJ03: 5'-ATTACAAATTTCACATTGCCACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8268445
   
wt amplicon (over InsPos) 04bg - BJ03
wt amplicon start Chr1:8268445
wt amplicon end Chr1:8269437
wt amplicon size 993 bp
Locus specific primer BJ03: 5'-ATTACAAATTTCACATTGCCACAC-3'
2nd locus specific primer 04bg: 5'-GATTATTACCTGTGCAGATGGGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11