Genotyping and primer details


Genotyping and primer details for 016D02 - At1g64280
Line ID 016D02
Locus At1g64280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23854446 of line 016D02 Chr1:23854446
   
Insertion allele amplicon 8474 - KY09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KY09: 5'-AGCGCTTTTAGTCTACTTTGATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23853731
   
wt amplicon (over InsPos) 11cm - KY09
wt amplicon start Chr1:23853731
wt amplicon end Chr1:23854916
wt amplicon size 1186 bp
Locus specific primer KY09: 5'-AGCGCTTTTAGTCTACTTTGATGC-3'
2nd locus specific primer 11cm: 5'-GGATTTCATGTTGGAGGTTCTCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11