SimpleSearch - Line and FST details
Line specific information
| Line ID | 016D02 |
| Vector Used | pAC106 |
| Line Availability | available as T3 set from NASC (N401478) |
| Segregation Analysis | 100:96:92 |
| Confirmed for Hit | At1g64280 At1g18130 |
| Parent of DUPLO pair | 2546 |
| Parent of pair(s) | none |
| Note |
Gene hit F13M14
| Sequence (A. th genome BLAST matches underlined) | >12-K012439-022-016-D02-8409 TTGTACATGTAGATTTCCCGGACTGAAGCACTGACATGTGGATTTCCCTGGGTATCGAAG GCGCGCGTGACCGACTGTGGGAGGCTCTGCTGCGAATACGCCGTATGACCCTGCGGACAC TAACATTTTTGAATTGAAAAAAAATTGGAATCACTCTTTTTTTTTGCCCTATTGACCATC ATACTCATTGTTGGACCTGGCTAGGCTAAGGACACGAGAGCCCTCTTGTCAAAATCGTGA GCGCTCTAGGAGACTTGGGACCTAATTCGAATGAGGACACGGGCACCGCGCCTATAGTGA CGGACAATTCTCAATTGAGTTTATACTTTGCTATACGTTGTTGATGTTCTTTTTGTCTTC ATTCTTAGAATTATCAGCACTCTTAACATGTACACAAACTCGCTAAGCTATGGGATCCTC CCTATAGGGAGTCGTATTACTC |
| GenBank Accession | AL752638 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:3282947 - go to primer design |
| BLAST e Value | 2e-43 |
| Hit Clone Code (BAC ID) | F13M14 |
| Insertion Classification | Genome Hit |
| Confirmation Status | failed |
Gene hit At1g64280
| Sequence (A. th genome BLAST matches underlined) | >10-K020161-022-016-D02t-8409 GAACTCTTCAAATACCAAAGACGTATGGGTATCCTCCCTATAGTGAGAAGANNNNNNTCC NAGGGGATATACGGGGCTTCATGTTGCTGCGATGCGGAAGGAGCCACAATTGATACTATC TCTATTGGAAAAAGGTGCAAGTGCATCAGAAGCAACTTTGGAAGGTAGAACCGCACTCAT GATCGCAAAACAAGCCACTATGGCGGTTGAATGTAATAATATCCCGGAGCAATGCAAGCA TTCTCTCAAAGGCCGACTATGTGTAGAAATACTATGGGATCCTCCCTATAGTGAG |
| GenBank Accession | BX530742 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:23854409 - go to primer design |
| BLAST e Value | 1e-115 |
| Hit Clone Code (BAC ID) | F15H21 |
| Hit Gene Code | At1g64280 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | regulatory protein (NPR1) |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At5g23630
| Sequence (A. th genome BLAST matches underlined) | >07-K028184-022-016-D02t-8409 AATATAAATTAGCTTCATACAATTGAACTCTTCAAATACCAAAGTCTAGAGAGCGGAACT TCAAAGAGAAGTCATATAACAAACAAAGATGATGTTAATGAAAAAAAAGTATGGGATCCT CCCTATAGTGAGTCGTATTACTCACCCTCTTTTACGCTTTCCTTTTCTGATATCGCTGCA GACCTCGCTGTCATTCCAGCGTCGCCGTTGCTGTCCTTGCATCTGATGATTACTTATCCA TCACAAGATATACGAATCATGTTTTATTTGGCGGGACCNCGATCTTGCGACATTCGCCTG ACAAGGTATTTCCGTCTCAGCGATATCGAGCATTTCCTTCTTC |
| GenBank Accession | CR769881 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:7965786 - go to primer design |
| BLAST e Value | 2e-35 |
| Hit Clone Code (BAC ID) | MQM1 |
| Hit Gene Code | At5g23630 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | phosphate deficiency response 2 |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | CR769881 [GenBank] |
Gene hit At1g18130
| Sequence (A. th genome BLAST matches underlined) | >11-K017011-022-016-D02T-8409 TGTCATGACATTTCCCGGACATGAGCCATTTACAATTGATATATCCTTGACATGCATGCC GAGACTATGGGATCCTCCCATATAGTGAGAACCATTGAGGTTTATGTTCCCGTATACATT TTGAATGATGATTGTGGTTGTGAGTAGGTGCAAGAAGCTCAGGTTGCACACTACAACTAC ATACTAGCCGTGGGTGATGAAGAGGCTATGGGATCCTCCCCTATAGTGAG |
| GenBank Accession | AL936019 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr1:6238478 - go to primer design |
| BLAST e Value | 2e-58 |
| Hit Clone Code (BAC ID) | T10F20 |
| Hit Gene Code | At1g18130 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Class II aaRS and biotin synthetases superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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