Genotyping and primer details


Genotyping and primer details for 026E07 - At2g34040
Line ID 026E07
Locus At2g34040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14379094 of line 026E07 Chr2:14379094
   
Insertion allele amplicon 8474 - 04sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04sf: 5'-ATCGTTGCCTTAGTTAGATCCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14378689
   
wt amplicon (over InsPos) 10tc - 04sf
wt amplicon start Chr2:14378689
wt amplicon end Chr2:14379418
wt amplicon size 730 bp
Locus specific primer 04sf: 5'-ATCGTTGCCTTAGTTAGATCCTCA-3'
2nd locus specific primer 10tc: 5'-AAGCACTTGCTGATATTTCTCCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11