Genotyping and primer details

Genotyping and primer details for 030F04 - At5g48410
Line ID 030F04
Locus At5g48410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19621787 of line 030F04 Chr5:19621787
Insertion allele amplicon 8474 - MF29
Locus specific primer MF29: 5'-AACTCTCCTTGAGGAAATCACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19621454
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11