Genotyping and primer details


Genotyping and primer details for 038C07 - At2g46400
Line ID 038C07
Locus At2g46400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19044434 of line 038C07 Chr2:19044434
   
Insertion allele amplicon 8474 - AJ06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AJ06: 5'-AAGATCCTTCTCTTTTCCACACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19044989
   
Insertion allele amplicon 8474 - IS25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IS25: 5'-AAGATCCTTCTCTTTTCCACACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19044989
   
wt amplicon (over InsPos) 00ab - AJ06
wt amplicon start Chr2:19044119
wt amplicon end Chr2:19044989
wt amplicon size 871 bp
Locus specific primer AJ06: 5'-AAGATCCTTCTCTTTTCCACACAA-3'
2nd locus specific primer 00ab: 5'-CTGTGTCTGATTTCTGAACTTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11