Genotyping and primer details


Genotyping and primer details for 039A05 - At1g76550
Line ID 039A05
Locus At1g76550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28724127 of line 039A05 Chr1:28724127
   
Insertion allele amplicon 8474 - FL80
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL80: 5'-GACAGATAGCGTTGAATTTCTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28723752
   
Insertion allele amplicon 8474 - RK79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK79: 5'-TTTTGGTATGTTGGAAGTGATGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28724544
   
wt amplicon (over InsPos) 04sq - RK79
wt amplicon start Chr1:28723587
wt amplicon end Chr1:28724544
wt amplicon size 958 bp
Locus specific primer RK79: 5'-TTTTGGTATGTTGGAAGTGATGTC-3'
2nd locus specific primer 04sq: 5'-TGGTAACATACATGTCCAAGAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11