Genotyping and primer details


Genotyping and primer details for 052D06 - At5g55090
Line ID 052D06
Locus At5g55090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22357002 of line 052D06 Chr5:22357002
   
Insertion allele amplicon 8474 - 60aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 60aq: 5'-GATAGAGATGGCTACAGGGTCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22357632
   
wt amplicon (over InsPos) 22au - 60aq
wt amplicon start Chr5:22356460
wt amplicon end Chr5:22357632
wt amplicon size 1173 bp
Locus specific primer 60aq: 5'-GATAGAGATGGCTACAGGGTCAAG-3'
2nd locus specific primer 22au: 5'-CATGGTAATCACTCACTTGCACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11