Genotyping and primer details


Genotyping and primer details for 055A02 - At5g56600
Line ID 055A02
Locus At5g56600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22910636 of line 055A02 Chr5:22910636
   
Insertion allele amplicon 8474 - FM26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FM26: 5'-CTTTTAGAACGTGCTGATAACGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22911230
   
Insertion allele amplicon 8474 - IN01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IN01: 5'-ACTTGTTACCACCAAGGAACAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22910130
   
wt amplicon (over InsPos) FM26 - IN01
wt amplicon start Chr5:22910130
wt amplicon end Chr5:22911230
wt amplicon size 1101 bp
Locus specific primer IN01: 5'-ACTTGTTACCACCAAGGAACAATC-3'
2nd locus specific primer FM26: 5'-CTTTTAGAACGTGCTGATAACGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11