Genotyping and primer details


Genotyping and primer details for 062F08 - At2g34170
Line ID 062F08
Locus At2g34170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14429630 of line 062F08 Chr2:14429630
   
Insertion allele amplicon 8474 - 04ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ve: 5'-ATTGTAATTCAACGTGATGATCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14430096
   
wt amplicon (over InsPos) 19vu - 04ve
wt amplicon start Chr2:14428920
wt amplicon end Chr2:14430096
wt amplicon size 1177 bp
Locus specific primer 04ve: 5'-ATTGTAATTCAACGTGATGATCCA-3'
2nd locus specific primer 19vu: 5'-GCTTTAAAAACTCACTCGGTATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11