Genotyping and primer details


Genotyping and primer details for 064E07 - At5g37300
Line ID 064E07
Locus At5g37300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14768161 of line 064E07 Chr5:14768161
   
Insertion allele amplicon 8474 - 01im
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01im: 5'-TATGCTAAGTTCGCCAAATTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14767585
   
wt amplicon (over InsPos) 17ji - 01im
wt amplicon start Chr5:14767585
wt amplicon end Chr5:14768550
wt amplicon size 966 bp
Locus specific primer 01im: 5'-TATGCTAAGTTCGCCAAATTTCTT-3'
2nd locus specific primer 17ji: 5'-TCCTCAAAACTTATTATCCGATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11