Genotyping and primer details


Genotyping and primer details for 068A01 - At1g74770
Line ID 068A01
Locus At1g74770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28089835 of line 068A01 Chr1:28089835
   
wt amplicon (over InsPos) 15tx - 03tg
wt amplicon start Chr1:28089283
wt amplicon end Chr1:28090384
wt amplicon size 1102 bp
Locus specific primer 03tg: 5'-GGGTATTGACTACTTCCACTGCAT-3'
2nd locus specific primer 15tx: 5'-GAACTCAGGCTGTTTTGTAGGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11