Genotyping and primer details


Genotyping and primer details for 072B11 - At1g14360
Line ID 072B11
Locus At1g14360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4911896 of line 072B11 Chr1:4911896
   
Insertion allele amplicon 8474 - RK40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK40: 5'-AGGTGAGGTGTCAATTTAGAGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4912269
   
wt amplicon (over InsPos) 08cu - RK40
wt amplicon start Chr1:4911569
wt amplicon end Chr1:4912269
wt amplicon size 701 bp
Locus specific primer RK40: 5'-AGGTGAGGTGTCAATTTAGAGCAT-3'
2nd locus specific primer 08cu: 5'-CAAGAAGATGAAGTTTTGGCCTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11