Genotyping and primer details


Genotyping and primer details for 075A07 - At5g66550
Line ID 075A07
Locus At5g66550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26560562 of line 075A07 Chr5:26560562
   
Insertion allele amplicon 8474 - 47ux
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 47ux: 5'-TCGTGGTATGTTCAAATTGTGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26560961
   
wt amplicon (over InsPos) 10vj - 47ux
wt amplicon start Chr5:26560176
wt amplicon end Chr5:26560961
wt amplicon size 786 bp
Locus specific primer 47ux: 5'-TCGTGGTATGTTCAAATTGTGATT-3'
2nd locus specific primer 10vj: 5'-ATCTCATGGAAATAAACCTGCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11