Genotyping and primer details


Genotyping and primer details for 080D06 - At3g07340
Line ID 080D06
Locus At3g07340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2342209 of line 080D06 Chr3:2342209
   
Insertion allele amplicon 8474 - PH63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH63: 5'-AATTGCAAATTTTTGTTGTGATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2341613
   
wt amplicon (over InsPos) 13fw - PH63
wt amplicon start Chr3:2341613
wt amplicon end Chr3:2342524
wt amplicon size 912 bp
Locus specific primer PH63: 5'-AATTGCAAATTTTTGTTGTGATGA-3'
2nd locus specific primer 13fw: 5'-GAAGATTCTGATCCAAAGAGATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11