Genotyping and primer details


Genotyping and primer details for 098E08 - At4g36790
Line ID 098E08
Locus At4g36790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17336486 of line 098E08 Chr4:17336486
   
Insertion allele amplicon 8474 - AN03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AN03: 5'-TACAACACCTCCTCCTATACCACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17337213
   
wt amplicon (over InsPos) AN04 - AN03
wt amplicon start Chr4:17336340
wt amplicon end Chr4:17337213
wt amplicon size 874 bp
Locus specific primer AN03: 5'-TACAACACCTCCTCCTATACCACC-3'
2nd locus specific primer AN04: 5'-AGTTCAAGTTTTGGATTTTGATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11