Genotyping and primer details


Genotyping and primer details for 104F11 - At1g72620
Line ID 104F11
Locus At1g72620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27342208 of line 104F11 Chr1:27342208
   
Insertion allele amplicon 3144 - 05vo
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 05vo: 5'-TTACAGCATTGCCAAATTATGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27342635
   
wt amplicon (over InsPos) 49vx - 05vo
wt amplicon start Chr1:27341619
wt amplicon end Chr1:27342635
wt amplicon size 1017 bp
Locus specific primer 05vo: 5'-TTACAGCATTGCCAAATTATGTTC-3'
2nd locus specific primer 49vx: 5'-ATTGTGAGTAGTGGAGTTGGGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11