Genotyping and primer details


Genotyping and primer details for 111E10 - At1g64300
Line ID 111E10
Locus At1g64300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23864901 of line 111E10 Chr1:23864901
   
Insertion allele amplicon 8474 - PH12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH12: 5'-GGGGATAGTTTTGTGTTGAGACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23864317
   
wt amplicon (over InsPos) 01mx - PH12
wt amplicon start Chr1:23864317
wt amplicon end Chr1:23865264
wt amplicon size 948 bp
Locus specific primer PH12: 5'-GGGGATAGTTTTGTGTTGAGACAT-3'
2nd locus specific primer 01mx: 5'-CATCTTCTCTTTCTCTGCAATCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11