Genotyping and primer details


Genotyping and primer details for 114A12 - At4g32980
Line ID 114A12
Locus At4g32980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15916018 of line 114A12 Chr4:15916018
   
Insertion allele amplicon 3144 - FI66
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FI66: 5'-ACTTCTTGTCAAGCCACTTCGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15915439
   
Insertion allele amplicon 3144 - MR50
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer MR50: 5'-TTTGGCTCAAAATACCTTCACTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15916267
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11