Genotyping and primer details


Genotyping and primer details for 120D06 - At3g21820
Line ID 120D06
Locus At3g21820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7689423 of line 120D06 Chr3:7689423
   
Insertion allele amplicon 3144 - IN41
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IN41: 5'-AATTATCACTGCTTGTCCGTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7688861
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11