Genotyping and primer details


Genotyping and primer details for 126H04 - At2g23760
Line ID 126H04
Locus At2g23760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10110125 of line 126H04 Chr2:10110125
   
Insertion allele amplicon 8474 - 13sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13sm: 5'-TGGCTATTCGAGCATTTTCTTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10110646
   
wt amplicon (over InsPos) 17ti - 13sm
wt amplicon start Chr2:10109619
wt amplicon end Chr2:10110646
wt amplicon size 1028 bp
Locus specific primer 13sm: 5'-TGGCTATTCGAGCATTTTCTTAAT-3'
2nd locus specific primer 17ti: 5'-CTAAGGGACAAAAGAAAAAGCTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11