Genotyping and primer details


Genotyping and primer details for 139A12 - At3g23810
Line ID 139A12
Locus At3g23810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8588186 of line 139A12 Chr3:8588186
   
Insertion allele amplicon 8474 - IF01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IF01: 5'-CTCATGAGGGCTACTGATGTTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8588717
   
wt amplicon (over InsPos) 14bj - IF01
wt amplicon start Chr3:8587702
wt amplicon end Chr3:8588717
wt amplicon size 1016 bp
Locus specific primer IF01: 5'-CTCATGAGGGCTACTGATGTTATG-3'
2nd locus specific primer 14bj: 5'-TTGCGTATTTTGTTTTCACTCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11