Genotyping and primer details


Genotyping and primer details for 143D07 - At1g16780
Line ID 143D07
Locus At1g16780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5740466 of line 143D07 Chr1:5740466
   
Insertion allele amplicon 8474 - FL20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FL20: 5'-TGAATTTCTTCATGTGTGGCTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5741080
   
Insertion allele amplicon 3144 - RK85
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer RK85: 5'-CCATAATCTGGCTTCTCCTTGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5740089
   
wt amplicon (over InsPos) RK85 - FL20
wt amplicon start Chr1:5740089
wt amplicon end Chr1:5741080
wt amplicon size 992 bp
Locus specific primer FL20: 5'-TGAATTTCTTCATGTGTGGCTTAG-3'
2nd locus specific primer RK85: 5'-CCATAATCTGGCTTCTCCTTGTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11