Genotyping and primer details

Genotyping and primer details for 144H10 - At5g42000
Line ID 144H10
Locus At5g42000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16799846 of line 144H10 Chr5:16799846
Insertion allele amplicon 8474 - 04qw
Locus specific primer 04qw: 5'-AGAAGAAAGAAAACTTGCTCAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16799374
wt amplicon (over InsPos) 07rm - 04qw
wt amplicon start Chr5:16799374
wt amplicon end Chr5:16800298
wt amplicon size 925 bp
Locus specific primer 04qw: 5'-AGAAGAAAGAAAACTTGCTCAACG-3'
2nd locus specific primer 07rm: 5'-GGAAACTTAGGTCCAACCCTAATC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11