Genotyping and primer details


Genotyping and primer details for 161D12 - At4g38380
Line ID 161D12
Locus At4g38380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17974291 of line 161D12 Chr4:17974291
   
Insertion allele amplicon 8474 - BM11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BM11: 5'-TCGAAGCTTTCTTTCTTTCTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17974868
   
wt amplicon (over InsPos) 01sl - BM11
wt amplicon start Chr4:17973990
wt amplicon end Chr4:17974868
wt amplicon size 879 bp
Locus specific primer BM11: 5'-TCGAAGCTTTCTTTCTTTCTTTTG-3'
2nd locus specific primer 01sl: 5'-AGATATAAGGCTGGGAAAAATTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11