Genotyping and primer details


Genotyping and primer details for 175D10 - At5g37800
Line ID 175D10
Locus At5g37800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15037127 of line 175D10 Chr5:15037127
   
Insertion allele amplicon 8474 - LD03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LD03: 5'-GATCTAGGTCTGTTTCTCGGGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15038122
   
wt amplicon (over InsPos) 04tc - LD03
wt amplicon start Chr5:15036664
wt amplicon end Chr5:15038122
wt amplicon size 1459 bp
Locus specific primer LD03: 5'-GATCTAGGTCTGTTTCTCGGGTAG-3'
2nd locus specific primer 04tc: 5'-TGGTTCAACTATTGTAAACATCGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11