Genotyping and primer details


Genotyping and primer details for 200C11 - At4g37670
Line ID 200C11
Locus At4g37670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17696690 of line 200C11 Chr4:17696690
   
Insertion allele amplicon 8474 - 13sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13sj: 5'-AGTGAGTGCTTTTTAAACGTTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17697095
   
wt amplicon (over InsPos) 10sz - 13sj
wt amplicon start Chr4:17696139
wt amplicon end Chr4:17697095
wt amplicon size 957 bp
Locus specific primer 13sj: 5'-AGTGAGTGCTTTTTAAACGTTGGT-3'
2nd locus specific primer 10sz: 5'-ATGGTTCTGTGTGTTTGCTTAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11