Genotyping and primer details


Genotyping and primer details for 202B03 - At4g30470
Line ID 202B03
Locus At4g30470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14895187 of line 202B03 Chr4:14895187
   
Insertion allele amplicon 8474 - 12et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12et: 5'-ACATGTAACGTTGTCTTCTGTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14894606
   
wt amplicon (over InsPos) 09fo - 12et
wt amplicon start Chr4:14894606
wt amplicon end Chr4:14895748
wt amplicon size 1143 bp
Locus specific primer 12et: 5'-ACATGTAACGTTGTCTTCTGTTGC-3'
2nd locus specific primer 09fo: 5'-ACAGTTTGTTAGCACGTGAGTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11