Genotyping and primer details


Genotyping and primer details for 225G05 - At4g31420
Line ID 225G05
Locus At4g31420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15247480 of line 225G05 Chr4:15247480
   
Insertion allele amplicon 8474 - 07br
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07br: 5'-TTACAAAAAGGCTAAAAGAATGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15248062
   
wt amplicon (over InsPos) 11cg - 07br
wt amplicon start Chr4:15246989
wt amplicon end Chr4:15248062
wt amplicon size 1074 bp
Locus specific primer 07br: 5'-TTACAAAAAGGCTAAAAGAATGGG-3'
2nd locus specific primer 11cg: 5'-CACCATCATCATCCATATCTTCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11