Genotyping and primer details


Genotyping and primer details for 243B11 - At5g45300
Line ID 243B11
Locus At5g45300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18356268 of line 243B11 Chr5:18356268
   
Insertion allele amplicon 8474 - AK09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AK09: 5'-TGTTCTTCCAAGAAAGAGGTGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18355837
   
wt amplicon (over InsPos) 01nv - AK09
wt amplicon start Chr5:18355837
wt amplicon end Chr5:18356581
wt amplicon size 745 bp
Locus specific primer AK09: 5'-TGTTCTTCCAAGAAAGAGGTGAAT-3'
2nd locus specific primer 01nv: 5'-CATGCATGCGTTTAATGAAGTAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11